Department of Medical Informatics
University Medical Center Göttingen
View primary antibody
Basic data
| PID | primary-0030 |
| EPIC PID | https://for2848.gwdguser.de/antibodies/primary-0030 |
| Research group | RG Möbius |
| Quality (mean) | no score (0.00) |
| Sharing level | Public |
Antibody data
| Antigen symbol | ACTA1 |
| Antibody Registry ID(s) | AB_2223041 |
| Name | Anti-Actin Antibody |
| Alternative name | NEM3 |
| Lab ID | |
| Tag / Fluorophore | |
| Raised in | Mouse |
| Reacts with | |
| Clone | C4 |
| Isotype | IgG1κ |
| Clonality | monoclonal |
| Demasking | unknown |
| Antigen | Purified chicken gizzard actin |
| Crafted By | company |
| Company / Manufacturer | Merck Millipore |
| Catalog no. | MAB1501 |
| Lot no. | |
| Description | Indirect immunofluorescence at 1:100: Tissue culture cells -- fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils -- fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6 µm) -- quick frozen in isopentane, slides treated with gelatin and formaldehyde. Immunoblots: 1:100-1:1,000 (Otey, 1987):On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle. Iodination (Lessard, 1979). Solid phase binding assay ELISA: 1:800-1:1,000 dilution from a previous lot was shown to be strongly reactive with cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum. ELISA: strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum. Optimal working dilutions must be determined by end user. |
| Localization | |
| Storage instruction | Stable at -20°C in undiluted aliquots for up to 12 months from date of receipt. Do not store in a diluted format. Avoid repeated freeze/thaw cycles. |
| Receipt date | 1905-07-14 00:00:00 |
| Preparation date | 1905-07-14 00:00:00 |
| Created by | , |
| Last modified | , |
External links
| Antibodypedia | - |
| HGNC | http://www.genenames.org/cgi-bin/gene_symbol_report?match=ACTA1 |
| Wikipedia | http://en.wikipedia.org/wiki/ACTA1 |
| Uniprot | http://www.uniprot.org/uniprot/?query=ACTA1 |
| GeneCards | http://www.genecards.org/cgi-bin/carddisp.pl?gene=ACTA1 |
| Antibody Registry | http://antibodyregistry.org/search?q=AB_2223041 |
Applications
No application comments yet.
Linked publications
There are no linked publications.
