View primary antibody

Basic data

Research groupRG Möbius
Quality (mean)no score (0.00)
Sharing levelPublic

Antibody data

Antigen symbolAIF1
Antibody Registry ID(s)AB_2224402
NameAnti-Iba1 antibody
Alternative nameinterferon gamma responsive transcript
Lab ID
Tag / Fluorophore
Raised inGoat
Reacts with
AntigenSynthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal)
Crafted Bycompany
Company / ManufacturerAbcam
Catalog no.ab5076
Lot no.
DescriptionIHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa). A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background.
LocalizationCytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
Storage instructionShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Receipt date1905-07-13 00:00:00
Preparation date1905-07-13 00:00:00
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Last modified,

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