View primary antibody

Basic data

PID primary-0029
EPIC PID
Research group RG Möbius
Quality (mean) no score (0.00)
Sharing level Public

Antibody data

Antigen symbol AIF1
Antibody Registry ID(s) AB_2224402
Name Anti-Iba1 antibody
Alternative name interferon gamma responsive transcript
Lab ID
Tag / Fluorophore
Raised in
Reacts with
Clone
Isotype IgG
Clonality polyclonal
Demasking unknown
Antigen Synthetic peptide corresponding to Human Iba1 aa 135-147 (C terminal)
Crafted By company
Company / Manufacturer Abcam
Catalog no. ab5076
Lot no.
Description IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 16-17 kDa (predicted molecular weight: 17 kDa). A 1 hour primary incubation is recommended for this product. We received several excellent Abreviews on this antibody working with mouse, however, mouse is not a batch tested species and we cannot guarantee that this antibody will work on mouse. Some of our customers have observed high background in mouse samples. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. Blocking with BSA gives high background.
Localization Cytoplasm > cytoskeleton. Cell projection > ruffle membrane. Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.
Storage instruction Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Receipt date 1905-07-13 00:00:00
Preparation date 1905-07-13 00:00:00
Created by
Last modified

External links

Antibodypedia -
HGNC https://www.genenames.org/tools/search/#!/?query=AIF1
Wikipedia https://en.wikipedia.org/wiki/AIF1
Uniprot https://www.uniprot.org/uniprot/?query=AIF1
GeneCards https://www.genecards.org/cgi-bin/carddisp.pl?gene=AIF1
Antibody Registry https://antibodyregistry.org/search?q=AB_2224402

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